› 1w0h

X-ray diffraction
1.59Å resolution

Crystallographic structure of the nuclease domain of 3'hExo, a DEDDh family member, bound to rAMP

Released:
DOI: 10.2210/pdb1w0h/pdb
PDB entry 1w0h coloured by chain, front view.
PDB entry 1w0h coloured by chain, side view.
PDB entry 1w0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic structure of the nuclease domain of 3'hExo, a DEDDh family member, bound to rAMP.
Cheng Y, Patel DJ
J Mol Biol 343 305-12 (2004)
PMID: 15451662

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3'-5' exoribonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 24.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IV48 (Residues: 120-322; Coverage: 58%)
Gene names: 3'EXO, ERI1, THEX1
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand AMP 1 x AMP
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P6122
Unit cell:
a: 101.6Å b: 101.6Å c: 100.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
7 more

5 mentions without citation

Crystallographic structure of the nuclease domain of 3'hExo, a DEDDh family member, bound to rAMP.
Cheng et al. (2004)
4 more