1vzw

X-ray diffraction
1.8Å resolution

Crystal structure of the bifunctional protein Pria

Released:

Function and Biology Details

Reactions catalysed:
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-147665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosyl isomerase A Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 25.5 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: P16250 (Residues: 1-240; Coverage: 100%)
Gene names: SC4G6.19c, SCO2050, hisA, priA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P3121
Unit cell:
a: 64.473Å b: 64.473Å c: 102.869Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.164 0.205
Expression system: Escherichia coli