› 1vlo

X-ray diffraction
1.7Å resolution

Crystal structure of aminomethyltransferase (T protein; tetrahydrofolate-dependent) of glycine cleavage system (np417381) from Escherichia coli k12 at 1.70 A resolution

Released:
DOI: 10.2210/pdb1vlo/pdb
PDB entry 1vlo coloured by chain, front view.
PDB entry 1vlo coloured by chain, side view.
PDB entry 1vlo coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminomethyltransferase Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 42.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P27248 (Residues: 2-364; Coverage: 100%)
Gene names: JW2873, b2905, gcvT
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 46.517Å b: 64.564Å c: 117.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.195
Expression system: Escherichia coli

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