› 1vet

X-ray diffraction
1.9Å resolution

Crystal Structure of p14/MP1 at 1.9 A resolution

Released:
DOI: 10.2210/pdb1vet/pdb
PDB entry 1vet coloured by chain, front view.
PDB entry 1vet coloured by chain, side view.
PDB entry 1vet coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes.
Kurzbauer R, Teis D, de Araujo ME, Maurer-Stroh S, Eisenhaber F, Bourenkov GP, Bartunik HD, Hekman M, Rapp UR, Huber LA, Clausen T
Proc Natl Acad Sci U S A 101 10984-9 (2004)
PMID: 15263099

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131570 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ragulator complex protein LAMTOR3 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O88653 (Residues: 1-124; Coverage: 100%)
Gene names: Lamtor3, Map2k1ip1, Mapbp, Mapksp1
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Structure domains: Dynein light chain 2a, cytoplasmic
Ragulator complex protein LAMTOR2 Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 13.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JHS3 (Residues: 1-125; Coverage: 100%)
Gene names: Lamtor2, Mapbpip, Robld3
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 50.078Å b: 63.289Å c: 72.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.259
Expression system: Escherichia coli

Quick links

Citations

20 review citations

ERK1/2 MAP kinases: structure, function, and regulation.
Roskoski R. (2012)
19 more

8 mentions without citation

Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II.
Levine et al. (2013)
7 more