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X-ray diffraction
2.01Å resolution

Crystal structure of the N-terminal domain of rck/p54, a human DEAD-box protein

Released:
DOI: 10.2210/pdb1vec/pdb
PDB entry 1vec coloured by chain, front view.
PDB entry 1vec coloured by chain, side view.
PDB entry 1vec coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight of human DEAD-box protein rck/p54 into its substrate recognition with conformational changes.
Matsui T, Hogetsu K, Usukura J, Sato T, Kumasaka T, Akao Y, Tanaka N
Genes Cells 11 439-52 (2006)
PMID: 16611246

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent RNA helicase DDX6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 23.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26196 (Residues: 94-299; Coverage: 43%)
Gene names: DDX6, HLR2, RCK
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 65.52Å b: 73.14Å c: 84.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.251
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The DHH1/RCKp54 family of helicases: an ancient family of proteins that promote translational silencing.
Presnyak et al. (2013)
4 more

5 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
4 more