› 1v9d

X-ray diffraction
2.6Å resolution

Crystal structure of the core FH2 domain of mouse mDia1

Released:
DOI: 10.2210/pdb1v9d/pdb
PDB entry 1v9d coloured by chain, front view.
PDB entry 1v9d coloured by chain, side view.
PDB entry 1v9d coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.
Shimada A, Nyitrai M, Vetter IR, Kühlmann D, Bugyi B, Narumiya S, Geeves MA, Wittinghofer A
Mol Cell 13 511-22 (2004)
PMID: 14992721

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein diaphanous homolog 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 340 amino acids
Theoretical weight: 39.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08808 (Residues: 826-1163; Coverage: 27%)
Gene names: Diap1, Diaph1
Sequence domains: Formin Homology 2 Domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 82.43Å b: 124.521Å c: 229.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.236 0.267
Expression system: Escherichia coli

Quick links

Citations

18 review citations

A nucleator arms race: cellular control of actin assembly.
Campellone et al. (2010)
17 more

9 mentions without citation

Towards a molecular understanding of the apicomplexan actin motor: on a road to novel targets for malaria remedies?
Kumpula et al. (2015)
8 more