› 1v7w

X-ray diffraction
1.6Å resolution

Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc

Released:
DOI: 10.2210/pdb1v7w/pdb
PDB entry 1v7w coloured by chain, front view.
PDB entry 1v7w coloured by chain, side view.
PDB entry 1v7w coloured by chain, top view.
Source organism: Vibrio proteolyticus
Primary publication:
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold.
Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, Fushinobu S
Structure 12 937-47 (2004)
PMID: 15274915

Function and Biology Details

Reaction catalysed: 
N,N'-diacetylchitobiose + phosphate = N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181161 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N,N'-diacetylchitobiose phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 807 amino acids
Theoretical weight: 91.29 KDa
Source organism: Vibrio proteolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76IQ9 (Residues: 1-801; Coverage: 100%)
Gene name: chbP
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NDG 1 x NDG
Ligand NAG 1 x NAG
Ligand CA 3 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 141.064Å b: 70.729Å c: 80.41Å
α: 90° β: 98.41° γ: 90°
R-values:
R R work R free
0.158 0.157 0.179
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
8 more

6 mentions without citation

Structural dissection of the reaction mechanism of cellobiose phosphorylase.
Hidaka et al. (2006)
5 more