› 1uw6

X-ray diffraction
2.2Å resolution

X-ray structure of acetylcholine binding protein (AChBP) in complex with nicotine

Released:
DOI: 10.2210/pdb1uw6/pdb
PDB entry 1uw6 coloured by chain, front view.
PDB entry 1uw6 coloured by chain, side view.
PDB entry 1uw6 coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures.
Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK
Neuron 41 907-14 (2004)
PMID: 15046723

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 211 amino acids
Theoretical weight: 24.03 KDa
Source organism: Lymnaea stagnalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P58154 (Residues: 19-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand NCT 20 x NCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 233Å b: 267.422Å c: 73.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.265
Expression system: Komagataella pastoris

Quick links

Citations

87 review citations

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque et al. (2009)
86 more

79 mentions without citation

Molecular dynamics simulations and drug discovery.
Durrant et al. (2011)
78 more