› 1uow

X-ray diffraction
1.04Å resolution

Calcium binding domain C2B

Released:
DOI: 10.2210/pdb1uow/pdb
PDB entry 1uow coloured by chain, front view.
PDB entry 1uow coloured by chain, side view.
PDB entry 1uow coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystallographic identification of Ca2+ and Sr2+ coordination sites in synaptotagmin I C2B domain.
Cheng Y, Sequeira SM, Malinina L, Tereshko V, Söllner TH, Patel DJ
Protein Sci 13 2665-72 (2004)
PMID: 15340165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptotagmin-1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.82 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21707 (Residues: 271-421; Coverage: 36%)
Gene name: Syt1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand GOL 1 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 54.55Å b: 54.55Å c: 103.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.166 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

How does synaptotagmin trigger neurotransmitter release?
Chapman ER. (2008)
3 more

10 mentions without citation

Drugging Membrane Protein Interactions.
Yin et al. (2016)
9 more