› 1uly

X-ray diffraction
2.5Å resolution

Crystal structure analysis of the ArsR homologue DNA-binding protein from P. horikoshii OT3

Released:
DOI: 10.2210/pdb1uly/pdb
PDB entry 1uly coloured by chain, front view.
PDB entry 1uly coloured by chain, side view.
PDB entry 1uly coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structure of the PH1932 protein, a unique archaeal ArsR type winged-HTH transcription factor from Pyrococcus horikoshii OT3.
Itou H, Yao M, Watanabe N, Tanaka I
Proteins 70 1631-4 (2008)
PMID: 18076033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH arsR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 23.08 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59595 (Residues: 1-192; Coverage: 100%)
Gene name: PH1932
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R32
Unit cell:
a: 90.64Å b: 90.64Å c: 132.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.268
Expression system: Escherichia coli

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Citations

2 review citations

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
1 more