› 1ujq

X-ray diffraction
2.1Å resolution

Crystal structure of 2-methylisocitrate lyase (PrpB) from Salmonella enterica serovar typhimurium

Released:
DOI: 10.2210/pdb1ujq/pdb
PDB entry 1ujq coloured by chain, front view.
PDB entry 1ujq coloured by chain, side view.
PDB entry 1ujq coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structure of Salmonella typhimurium 2-methylisocitrate lyase (PrpB) and its complex with pyruvate and Mg(2+).
Simanshu DK, Satheshkumar PS, Savithri HS, Murthy MR
Biochem Biophys Res Commun 311 193-201 (2003)
PMID: 14575713

Function and Biology Details

Reaction catalysed: 
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-methylisocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 33.26 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56062 (Residues: 2-295; Coverage: 100%)
Gene names: STM0368, prpB
Sequence domains: Phosphoenolpyruvate phosphomutase
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 62.812Å b: 99.086Å c: 201.571Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.232
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis.
Gould et al. (2006)
10 more