› 1uim

X-ray diffraction
2.15Å resolution

Crystal Structure of Threonine Synthase from Thermus Thermophilus HB8, Orthorhombic Crystal Form

Released:
DOI: 10.2210/pdb1uim/pdb
PDB entry 1uim coloured by chain, front view.
PDB entry 1uim coloured by chain, side view.
PDB entry 1uim coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of threonine synthase from Thermus thermophilus HB8: conformational change, substrate recognition, and mechanism.
Omi R, Goto M, Miyahara I, Mizuguchi H, Hayashi H, Kagamiyama H, Hirotsu K
J Biol Chem 278 46035-45 (2003)
PMID: 12952961

Function and Biology Details

Reaction catalysed: 
O-phospho-L-homoserine + H(2)O = L-threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 37.05 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83823 (Residues: 1-351; Coverage: 100%)
Gene name: TthHB5018_04960
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 69.068Å b: 83.271Å c: 123.504Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.251
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The aspartic acid metabolic pathway, an exciting and essential pathway in plants.
Azevedo et al. (2006)
2 more

2 mentions without citation

Product-assisted catalysis as the basis of the reaction specificity of threonine synthase.
Murakawa et al. (2011)
1 more