Function and Biology Details
Reaction catalysed:
An aliphatic amide = a nitrile + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Nitrile hydratase, beta subunit
- Nitrile hydratase alpha /Thiocyanate hydrolase gamma superfamily
- Nitrile hydratase beta subunit, N-terminal
- Nitrile hydratase beta subunit domain
- Electron transport accessory-like domain superfamily
- Nitrile hydratase, alpha subunit
- Nitrile hydratase alpha /Thiocyanate hydrolase gamma
- Nitrile hydratase alpha subunit /Thiocyanate hydrolase gamma subunit
Structure domains:
Structure analysis Details
Assemblies composition:
Assembly name:
Cobalt-containing nitrile hydratase (preferred)
PDBe Complex ID:
PDB-CPX-181955 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cobalt-containing nitrile hydratase subunit alpha
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.04 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
Structure domains: Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Length: 203 amino acids
Theoretical weight: 23.04 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
- Canonical:
Q7SID2 (Residues: 2-204; Coverage: 100%)
Structure domains: Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Cobalt-containing nitrile hydratase subunit beta
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 26.17 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
Structure domains:
Length: 228 amino acids
Theoretical weight: 26.17 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
- Canonical: Q7SID3 (Residues: 1-228; Coverage: 98%)
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SPRING-8 BEAMLINE BL40B2
Spacegroup:
P3221
Expression system: Escherichia coli
