› 1u56

X-ray diffraction
1.9Å resolution

Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (Water-ligated, ferric form)

Released:
DOI: 10.2210/pdb1u56/pdb
PDB entry 1u56 coloured by chain, front view.
PDB entry 1u56 coloured by chain, side view.
PDB entry 1u56 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases.
Pellicena P, Karow DS, Boon EM, Marletta MA, Kuriyan J
Proc Natl Acad Sci U S A 101 12854-9 (2004)
PMID: 15326296

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 22.05 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 91.61Å b: 127.18Å c: 42.59Å
α: 90° β: 95.48° γ: 90°
R-values:
R R work R free
0.225 0.225 0.255
Expression system: Escherichia coli

Quick links

Citations

43 review citations

NO-independent stimulators and activators of soluble guanylate cyclase: discovery and therapeutic potential.
Evgenov et al. (2006)
42 more

4 mentions without citation

Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases.
Pellicena et al. (2004)
3 more