› 1u3z

X-ray diffraction
1.9Å resolution

Crystal structure of MLAC mutant of dimerisation domain of NF-kB p50 transcription factor

Released:
DOI: 10.2210/pdb1u3z/pdb
PDB entry 1u3z coloured by chain, front view.
PDB entry 1u3z coloured by chain, side view.
PDB entry 1u3z coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Snapshot of protein structure evolution reveals conservation of functional dimerization through intertwined folding.
Chirgadze DY, Demydchuk M, Becker M, Moran S, Paoli M
Structure 12 1489-94 (2004)
PMID: 15296742

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclear factor NF-kappa-B p50 subunit Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 12.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25799 (Residues: 245-350; Coverage: 11%)
Gene name: Nfkb1
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P41212
Unit cell:
a: 63.097Å b: 63.097Å c: 64.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Genome reading by the NF-κB transcription factors.
Mulero et al. (2019)
1 more