› 1tyd

X-ray diffraction
2.5Å resolution

STRUCTURE OF TYROSYL-TRNA SYNTHETASE REFINED AT 2.3 ANGSTROMS RESOLUTION. INTERACTION OF THE ENZYME WITH THE TYROSYL ADENYLATE INTERMEDIATE

Released:
DOI: 10.2210/pdb1tyd/pdb
PDB entry 1tyd coloured by chain, front view.
PDB entry 1tyd coloured by chain, side view.
PDB entry 1tyd coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of tyrosyl-tRNA synthetase refined at 2.3 A resolution. Interaction of the enzyme with the tyrosyl adenylate intermediate.
Brick P, Bhat TN, Blow DM
J Mol Biol 208 83-98 (1989)
PMID: 2504923

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: E
Molecule details ›
Chain: E
Length: 319 amino acids
Theoretical weight: 36.31 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00952 (Residues: 1-319; Coverage: 76%)
Gene name: tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 1 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 64.46Å b: 64.46Å c: 237.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 not available not available
Expression system: Not provided

Quick links

Citations

27 review citations

Aminoacyl-tRNA synthesis.
Ibba et al. (2000)
26 more

4 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
3 more