› 1tw5

X-ray diffraction
2.3Å resolution

beta1,4-galactosyltransferase mutant M344H-Gal-T1 in complex with Chitobiose

Released:
DOI: 10.2210/pdb1tw5/pdb
PDB entry 1tw5 coloured by chain, front view.
PDB entry 1tw5 coloured by chain, side view.
PDB entry 1tw5 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose.
Ramakrishnan B, Boeggeman E, Qasba PK
Biochemistry 43 12513-22 (2004)
PMID: 15449940

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-galactose + D-glucose = UDP + lactose
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Processed beta-1,4-galactosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.86 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08037 (Residues: 130-402; Coverage: 68%)
Gene names: B4GALT1, GALT, GGTB2
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand MN 2 x MN
Ligand UDH 2 x UDH
Ligand SO4 5 x SO4
Ligand DIO 2 x DIO
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.37Å b: 92.55Å c: 143.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.257
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Substrate-induced conformational changes in glycosyltransferases.
Qasba et al. (2005)
5 more

3 mentions without citation

Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.
Moremen et al. (2019)
2 more