› 1tr1

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF E96K MUTATED BETA-GLUCOSIDASE A FROM BACILLUS POLYMYXA, AN ENZYME WITH INCREASED THERMORESISTANCE

Released:
DOI: 10.2210/pdb1tr1/pdb
PDB entry 1tr1 coloured by chain, front view.
PDB entry 1tr1 coloured by chain, side view.
PDB entry 1tr1 coloured by chain, top view.
Source organism: Paenibacillus polymyxa
Primary publication:
Crystal structure of beta-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases.
Sanz-Aparicio J, Hermoso JA, Martínez-Ripoll M, Lequerica JL, Polaina J
J Mol Biol 275 491-502 (1998)
PMID: 9466926

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 447 amino acids
Theoretical weight: 51.57 KDa
Source organism: Paenibacillus polymyxa
Expression system: Escherichia coli
UniProt:
  • Canonical: P22073 (Residues: 2-448; Coverage: 100%)
Gene name: bglA
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P42212
Unit cell:
a: 205.03Å b: 205.03Å c: 155.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.282
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Microbial beta-glucosidases: cloning, properties, and applications.
Bhatia et al. (2002)
6 more

4 mentions without citation

Detection of trans-cis flips and peptide-plane flips in protein structures.
Touw et al. (2015)
3 more