› 1tpy

X-ray diffraction
2.2Å resolution

Structure of the cyclopropane synthase MmaA2 from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1tpy/pdb
PDB entry 1tpy coloured by chain, front view.
PDB entry 1tpy coloured by chain, side view.
PDB entry 1tpy coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Smith CV, Sacchettini JC

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclopropane mycolic acid synthase MmaA2 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 32.76 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79FX6 (Residues: 1-287; Coverage: 100%)
Gene names: Rv0644c, mma2, mmaA2
Sequence domains: Mycolic acid cyclopropane synthetase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
2 bound ligands:
Ligand CO3 1 x CO3
Ligand 16A 1 x 16A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 42.587Å b: 58.42Å c: 133.683Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.194 0.232
Expression system: Escherichia coli BL21(DE3)

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