› 1tlw

X-ray diffraction
3.1Å resolution

Tsx structure complexed with thymidine

Released:
DOI: 10.2210/pdb1tlw/pdb
PDB entry 1tlw coloured by chain, front view.
PDB entry 1tlw coloured by chain, side view.
PDB entry 1tlw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the bacterial nucleoside transporter Tsx.
Ye J, van den Berg B
EMBO J 23 3187-95 (2004)
PMID: 15272310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside-specific channel-forming protein Tsx Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 32.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A927 (Residues: 23-294; Coverage: 100%)
Gene names: JW0401, b0411, nupA, tsx
Sequence domains: Nucleoside-specific channel-forming protein, Tsx
Structure domains: Nucleoside-specific channel-forming protein, Tsx-like

Ligands and Environments

1 bound ligand:
Ligand THM 4 x THM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 149.653Å b: 149.653Å c: 119.939Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 0.269 0.297
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria.
Vergalli et al. (2020)
9 more

6 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
5 more