› 1tdr

X-ray diffraction
2.5Å resolution

EXPRESSION, CHARACTERIZATION, AND CRYSTALLOGRAPHIC ANALYSIS OF TELLUROMETHIONYL DIHYDROFOLATE REDUCTASE

Released:
DOI: 10.2210/pdb1tdr/pdb
PDB entry 1tdr coloured by chain, front view.
PDB entry 1tdr coloured by chain, side view.
PDB entry 1tdr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Expression, characterization and crystallographic analysis of telluromethionyl dihydrofolate reductase.
Boles JO, Lewinski K, Kunckle MG, Hatada M, Lebioda L, Dunlap RB, Odom JD
Acta Crystallogr D Biol Crystallogr 51 731-9 (1995)
PMID: 15299803

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand MTX 2 x MTX
Ligand TE 4 x TE
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P61
Unit cell:
a: 93Å b: 93Å c: 74.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.124 not available not available
Expression system: Not provided

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Citations

1 citations in other articles

PRODRG, a program for generating molecular topologies and unique molecular descriptors from coordinates of small molecules.
van Aalten et al. (1996)