› 1tdf

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN REDUCTASE REFINED AT 2 ANGSTROM RESOLUTION: IMPLICATIONS FOR A LARGE CONFORMATIONAL CHANGE DURING CATALYSIS

Released:
DOI: 10.2210/pdb1tdf/pdb
PDB entry 1tdf coloured by chain, front view.
PDB entry 1tdf coloured by chain, side view.
PDB entry 1tdf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli thioredoxin reductase refined at 2 A resolution. Implications for a large conformational change during catalysis.
Waksman G, Krishna TS, Williams CH, Kuriyan J
J Mol Biol 236 800-16 (1994)
PMID: 8114095

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.13 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A9P4 (Residues: 2-317; Coverage: 98%)
Gene names: JW0871, b0888, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6322
Unit cell:
a: 123.8Å b: 123.8Å c: 81.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.179 not available
Expression system: Not provided

Quick links

Citations

15 review citations

Thioredoxin reductase.
Mustacich et al. (2000)
14 more

10 mentions without citation

In silico protein design by combinatorial assembly of protein building blocks.
Tsai et al. (2004)
9 more