› 1tde

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN REDUCTASE REFINED AT 2 ANGSTROM RESOLUTION: IMPLICATIONS FOR A LARGE CONFORMATIONAL CHANGE DURING CATALYSIS

Released:
DOI: 10.2210/pdb1tde/pdb
PDB entry 1tde coloured by chain, front view.
PDB entry 1tde coloured by chain, side view.
PDB entry 1tde coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli thioredoxin reductase refined at 2 A resolution. Implications for a large conformational change during catalysis.
Waksman G, Krishna TS, Williams CH, Kuriyan J
J Mol Biol 236 800-16 (1994)
PMID: 8114095

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.14 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A9P4 (Residues: 2-317; Coverage: 98%)
Gene names: JW0871, b0888, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6322
Unit cell:
a: 123.8Å b: 123.8Å c: 81.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 not available
Expression system: Not provided

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Citations

15 review citations

Thioredoxin reductase.
Mustacich et al. (2000)
14 more

16 mentions without citation

Protein hydration in solution: experimental observation by x-ray and neutron scattering.
Svergun et al. (1998)
15 more