› 1t9j

X-ray diffraction
2Å resolution

I-CreI(Q47E)/DNA complex

Released:
DOI: 10.2210/pdb1t9j/pdb
PDB entry 1t9j coloured by chain, front view.
PDB entry 1t9j coloured by chain, side view.
PDB entry 1t9j coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Metal-dependent DNA cleavage mechanism of the I-CreI LAGLIDADG homing endonuclease.
Chevalier B, Sussman D, Otis C, Noël AJ, Turmel M, Lemieux C, Stephens K, Monnat RJ, Stoddard BL
Biochemistry 43 14015-26 (2004)
PMID: 15518550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114636 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.72 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 1-163; Coverage: 100%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 43.11Å b: 67.86Å c: 87.65Å
α: 90° β: 91.55° γ: 90°
R-values:
R R work R free
0.238 0.238 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity.
Yang et al. (2006)
7 more

3 mentions without citation

Energetics of the protein-DNA-water interaction.
Spyrakis et al. (2007)
2 more