› 1sv1

Solution NMR

NMR structure of the ThKaiA180C-CIIABD complex (25-structure ensemble)

Released:
DOI: 10.2210/pdb1sv1/pdb
PDB entry 1sv1 coloured by chain, ensemble of 25 models, front view.
PDB entry 1sv1 coloured by chain, ensemble of 25 models, side view.
PDB entry 1sv1 coloured by chain, ensemble of 25 models, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: implications for KaiC regulation.
Vakonakis I, LiWang AC
Proc Natl Acad Sci U S A 101 10925-30 (2004)
PMID: 15256595

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181291 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Circadian clock oscillator protein KaiA Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.6 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79V62 (Residues: 180-283; Coverage: 37%)
Gene names: kaiA, tlr0481
Sequence domains: KaiA C-terminal domain
Structure domains: KaiA/RbsU domain
Circadian clock oscillator protein KaiC Chains: C, D
Molecule details ›
Chains: C, D
Length: 34 amino acids
Theoretical weight: 3.62 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79V60 (Residues: 488-518; Coverage: 6%)
Gene names: kaiC, tlr0483

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Distance geometry, Simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

The cyanobacterial circadian system: from biophysics to bioevolution.
Johnson et al. (2011)
18 more

3 mentions without citation

Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: implications for KaiC regulation.
Vakonakis et al. (2004)
2 more