› 1so0

X-ray diffraction
2.3Å resolution

Crystal structure of human galactose mutarotase complexed with galactose

Released:
DOI: 10.2210/pdb1so0/pdb
PDB entry 1so0 coloured by chain, front view.
PDB entry 1so0 coloured by chain, side view.
PDB entry 1so0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular structure of human galactose mutarotase.
Thoden JB, Timson DJ, Reece RJ, Holden HM
J Biol Chem 279 23431-7 (2004)
PMID: 15026423

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose = beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose mutarotase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 344 amino acids
Theoretical weight: 38.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96C23 (Residues: 1-342; Coverage: 100%)
Gene names: BLOCK25, GALM
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

1 bound ligand:
Ligand GAL 4 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 61Å b: 68.7Å c: 98.9Å
α: 107.7° β: 98.4° γ: 102.7°
R-values:
R R work R free
0.169 0.167 0.198
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Lipid and Carbohydrate Metabolism in Caenorhabditis elegans.
Watts et al. (2017)
2 more

3 mentions without citation

The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism.
Bakolitsa et al. (2010)
2 more