› 1sa0

X-ray diffraction
3.58Å resolution

TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX

Released:
DOI: 10.2210/pdb1sa0/pdb
PDB entry 1sa0 coloured by chain, front view.
PDB entry 1sa0 coloured by chain, side view.
PDB entry 1sa0 coloured by chain, top view.
Source organisms:
Primary publication:
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain.
Ravelli RB, Gigant B, Curmi PA, Jourdain I, Lachkar S, Sobel A, Knossow M
Nature 428 198-202 (2004)
PMID: 15014504

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-158932 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1D chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.16 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2HJ86 (Residues: 1-452; Coverage: 100%)
Gene name: TUBA1D
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 48-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand GTP 2 x GTP
Ligand GDP 2 x GDP
Ligand CN2 2 x CN2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 328.69Å b: 328.691Å c: 54.679Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.232 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

92 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
91 more

262 mentions without citation

An overview of tubulin inhibitors that interact with the colchicine binding site.
Lu et al. (2012)
261 more