› 1s96

X-ray diffraction
2Å resolution

The 2.0 A X-ray structure of Guanylate Kinase from E.coli

Released:
DOI: 10.2210/pdb1s96/pdb
PDB entry 1s96 coloured by chain, front view.
PDB entry 1s96 coloured by chain, side view.
PDB entry 1s96 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Kreusch A, Spraggon G, Klock HE, McMullan D, Vincent J, Rodrigues K, Lesley SA

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-158040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 25.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60546 (Residues: 1-207; Coverage: 100%)
Gene names: JW3623, b3648, gmk, spoR
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand UNX 1 x UNX
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P213
Unit cell:
a: 124.552Å b: 124.552Å c: 124.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.247
Expression system: Escherichia coli

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