› 1s85

X-ray diffraction
2.2Å resolution

PORCINE TRYPSIN COMPLEXED WITH P-HYDROXYMETHYL BENZAMIDINE AND BORATE

Released:
DOI: 10.2210/pdb1s85/pdb
PDB entry 1s85 coloured by chain, front view.
PDB entry 1s85 coloured by chain, side view.
PDB entry 1s85 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
X-ray and NMR characterization of covalent complexes of trypsin, borate, and alcohols.
Transue TR, Krahn JM, Gabel SA, DeRose EF, London RE
Biochemistry 43 2829-39 (2004)
PMID: 15005618

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.49 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00761 (Residues: 9-231; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand SO4 1 x SO4
Ligand SBZ 1 x SBZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 75.844Å b: 55.41Å c: 46.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.254

Quick links

Citations

2 review citations

In mesopore protein digestion: a new forthcoming strategy in proteomics.
Savino et al. (2011)
1 more

1 mention without citation

Structure basis 1/2SLPI and porcine pancreas trypsin interaction.
Fukushima et al. (2013)