› 1s7c

X-ray diffraction
2.04Å resolution

Crystal structure of MES buffer bound form of glyceraldehyde 3-phosphate dehydrogenase from Escherichia coli

Released:
DOI: 10.2210/pdb1s7c/pdb
PDB entry 1s7c coloured by chain, front view.
PDB entry 1s7c coloured by chain, side view.
PDB entry 1s7c coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Shin DH, Thor J, Yokota H, Kim R, Kim SH, Berkeley Structural Genomics Center (BSGC)

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase A Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 35.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9B2 (Residues: 1-331; Coverage: 100%)
Gene names: JW1768, b1779, gapA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I4122
Unit cell:
a: 121.511Å b: 121.511Å c: 156.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.22
Expression system: Escherichia coli

Quick links