› 1s6a

X-ray diffraction
1.69Å resolution

The X-ray structure of the cyanobacteria Synechocystis hemoglobin "cyanoglobin" with azide ligand

Released:
DOI: 10.2210/pdb1s6a/pdb
PDB entry 1s6a coloured by chain, front view.
PDB entry 1s6a coloured by chain, side view.
PDB entry 1s6a coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Crystallographic analysis of synechocystis cyanoglobin reveals the structural changes accompanying ligand binding in a hexacoordinate hemoglobin.
Trent JT, Kundu S, Hoy JA, Hargrove MS
J Mol Biol 341 1097-108 (2004)
PMID: 15289104

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 1 truncated hemoglobin GlbN Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.89 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73925 (Residues: 1-124; Coverage: 100%)
Gene names: glbN, slr2097
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand FLC 1 x FLC
Ligand AZI 1 x AZI
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I41
Unit cell:
a: 83.35Å b: 83.35Å c: 65.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.197 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Structure and reactivity of hexacoordinate hemoglobins.
Kakar et al. (2010)
3 more