› 1s67

X-ray diffraction
1.5Å resolution

Crystal structure of heme domain of direct oxygen sensor from E. coli

Released:
DOI: 10.2210/pdb1s67/pdb
PDB entry 1s67 coloured by chain, front view.
PDB entry 1s67 coloured by chain, side view.
PDB entry 1s67 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Insights into signal transduction involving PAS domain oxygen-sensing heme proteins from the X-ray crystal structure of Escherichia coli Dos heme domain (Ec DosH).
Park H, Suquet C, Satterlee JD, Kang C
Biochemistry 43 2738-46 (2004)
PMID: 15005609

Function and Biology Details

Reaction catalysed: 
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxygen sensor protein DosP Chains: L, U
Molecule details ›
Chains: L, U
Length: 119 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P76129 (Residues: 8-126; Coverage: 15%)
Gene names: JW1484, b1489, dos, dosP, pdeO, yddU
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 47.575Å b: 67.69Å c: 82.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.197 0.214
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Ligand-binding PAS domains in a genomic, cellular, and structural context.
Henry et al. (2011)
13 more

1 mention without citation

A conserved helical capping hydrogen bond in PAS domains controls signaling kinetics in the superfamily prototype photoactive yellow protein.
Kumauchi et al. (2010)