› 1s5j

X-ray diffraction
2.4Å resolution

Insight in DNA Replication: The crystal structure of DNA Polymerase B1 from the archaeon Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb1s5j/pdb
PDB entry 1s5j coloured by chain, front view.
PDB entry 1s5j coloured by chain, side view.
PDB entry 1s5j coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Insights into DNA replication: the crystal structure of DNA polymerase B1 from the archaeon Sulfolobus solfataricus.
Savino C, Federici L, Johnson KA, Vallone B, Nastopoulos V, Rossi M, Pisani FM, Tsernoglou D
Structure 12 2001-8 (2004)
PMID: 15530364

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150855 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase 1 Chain: A
Molecule details ›
Chain: A
Length: 847 amino acids
Theoretical weight: 97.53 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26811 (Residues: 36-882; Coverage: 96%)
Gene names: SSO0552, dpo1, polS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 9 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 187.47Å b: 68.8Å c: 125.85Å
α: 90° β: 107.94° γ: 90°
R-values:
R R work R free
0.264 0.232 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus.
Lipps G. (2006)
1 more

9 mentions without citation

DNA replication in the archaea.
Barry et al. (2006)
8 more