Function and Biology Details
Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assemblies composition:
Assembly name:
RNA-directed RNA polymerase (preferred)
PDBe Complex ID:
PDB-CPX-148695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase
Molecule details ›
Chains: A, B, C, D
Length: 601 amino acids
Theoretical weight: 68.95 KDa
Source organism: Bovine viral diarrhea virus 1
Expression system: Escherichia coli
UniProt:
Structure domains: Alpha-Beta Plaits
Length: 601 amino acids
Theoretical weight: 68.95 KDa
Source organism: Bovine viral diarrhea virus 1
Expression system: Escherichia coli
UniProt:
- Canonical:
P19711 (Residues: 3348-3948; Coverage: 15%)
Structure domains: Alpha-Beta Plaits
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 14-ID-B
Spacegroup:
P6222
Expression system: Escherichia coli
