› 1s00

X-ray diffraction
2.6Å resolution

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE

Released:
DOI: 10.2210/pdb1s00/pdb
PDB entry 1s00 coloured by chain, front view.
PDB entry 1s00 coloured by chain, side view.
PDB entry 1s00 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways.
Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G
Structure 12 703-15 (2004)
PMID: 15062092

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chains: L, R
Molecule details ›
Chains: L, R
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chains: M, S
Molecule details ›
Chains: M, S
Length: 307 amino acids
Theoretical weight: 34.34 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chains: H, T
Molecule details ›
Chains: H, T
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand BCL 8 x BCL
Ligand BPH 4 x BPH
Ligand U10 4 x U10
Ligand FE2 2 x FE2
Ligand SPO 2 x SPO
Ligand LDA 1 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 137.731Å b: 137.731Å c: 277.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.268
Expression system: Cereibacter sphaeroides

Quick links

Citations

4 review citations

Insights into the structure and function of HV1 from a meta-analysis of mutation studies.
DeCoursey et al. (2016)
3 more