› 1rzv

X-ray diffraction
2.3Å resolution

Crystal structure of the glycogen synthase from Agrobacterium tumefaciens (non-complexed form)

Released:
DOI: 10.2210/pdb1rzv/pdb
PDB entry 1rzv coloured by chain, front view.
PDB entry 1rzv coloured by chain, side view.
PDB entry 1rzv coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation.
Buschiazzo A, Ugalde JE, Guerin ME, Shepard W, Ugalde RA, Alzari PM
EMBO J 23 3196-205 (2004)
PMID: 15272305

Function and Biology Details

Reaction catalysed: 
ADP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = ADP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 485 amino acids
Theoretical weight: 52.93 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Agrobacterium tumefaciens
UniProt:
  • Canonical: P0A3F3 (Residues: 1-480; Coverage: 100%)
Gene names: glgA, glgA1
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 69.271Å b: 87.289Å c: 88.492Å
α: 90° β: 100.29° γ: 90°
R-values:
R R work R free
0.224 0.204 0.24
Expression system: Agrobacterium tumefaciens

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Citations

16 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
15 more

1 mention without citation

Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation.
Buschiazzo et al. (2004)