› 1rve

X-ray diffraction
2.5Å resolution

THE CRYSTAL STRUCTURE OF ECORV ENDONUCLEASE AND OF ITS COMPLEXES WITH COGNATE AND NON-COGNATE DNA FRAGMENTS

Released:
DOI: 10.2210/pdb1rve/pdb
PDB entry 1rve coloured by chain, front view.
PDB entry 1rve coloured by chain, side view.
PDB entry 1rve coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.
Winkler FK, Banner DW, Oefner C, Tsernoglou D, Brown RS, Heathman SP, Bryan RK, Martin PD, Petratos K, Wilson KS
EMBO J 12 1781-95 (1993)
PMID: 8491171

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137618 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme EcoRV Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 28.69 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P04390 (Residues: 1-245; Coverage: 100%)
Gene name: ecoRVR
Sequence domains: Restriction endonuclease EcoRV
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 58.2Å b: 71.7Å c: 130.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Not provided

Quick links

Citations

31 review citations

How do site-specific DNA-binding proteins find their targets?
Halford et al. (2004)
30 more

6 mentions without citation

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
5 more