› 1r8k

X-ray diffraction
2.1Å resolution

PDXA PROTEIN; NAD-DEPENDENT DEHYDROGENASE/CARBOXYLASE; SUBUNIT OF PYRIDOXINE PHOSPHATE BIOSYNTHETIC PROTEIN PDXJ-PDXA [SALMONELLA TYPHIMURIUM]

Released:
DOI: 10.2210/pdb1r8k/pdb
PDB entry 1r8k coloured by chain, front view.
PDB entry 1r8k coloured by chain, side view.
PDB entry 1r8k coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structure of NAD-dependent dehydrogenase/carboxylase of Salmonella typhimurium
Osipiuk J, Quartey P, Moy S, Collart F, Joachimiak A
To be published

Function and Biology Details

Reaction catalysed: 
4-phosphooxy-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxythreonine-4-phosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 35.14 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P58717 (Residues: 1-329; Coverage: 100%)
Gene names: STM0091, pdxA
Sequence domains: Pyridoxal phosphate biosynthetic protein PdxA
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

3 bound ligands:
Ligand CO 2 x CO
Ligand CL 1 x CL
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 119.954Å b: 119.954Å c: 55.541Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.234
Expression system: Escherichia coli

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