› 1r8c

X-ray diffraction
1.9Å resolution

Crystal Structures of an Archaeal Class I CCA-Adding Enzyme and Its Nucleotide

Released:
DOI: 10.2210/pdb1r8c/pdb
PDB entry 1r8c coloured by chain, front view.
PDB entry 1r8c coloured by chain, side view.
PDB entry 1r8c coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structures of an archaeal class I CCA-adding enzyme and its nucleotide complexes.
Xiong Y, Li F, Wang J, Weiner AM, Steitz TA
Mol Cell 12 1165-72 (2003)
PMID: 14636575

Function and Biology Details

Reaction catalysed: 
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCA-adding enzyme Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 51.47 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 5 x MN
Ligand NA 1 x NA
Ligand UTP 2 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 86.151Å b: 79.954Å c: 78.145Å
α: 90° β: 97.53° γ: 90°
R-values:
R R work R free
0.182 0.181 0.215
Expression system: Escherichia coli

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Citations

10 review citations

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
9 more