› 1r71

X-ray diffraction
2.2Å resolution

Crystal Structure of the DNA binding domain of KorB in complex with the operator DNA

Released:
DOI: 10.2210/pdb1r71/pdb
PDB entry 1r71 coloured by chain, front view.
PDB entry 1r71 coloured by chain, side view.
PDB entry 1r71 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Sequence-specific DNA binding determined by contacts outside the helix-turn-helix motif of the ParB homolog KorB.
Khare D, Ziegelin G, Lanka E, Heinemann U
Nat Struct Mol Biol 11 656-63 (2004)
PMID: 15170177

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114501 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional repressor protein KorB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 178 amino acids
Theoretical weight: 19.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07674 (Residues: 117-294; Coverage: 50%)
Gene name: korB
Sequence domains:
Structure domains:
5'-D(*AP*(BRU)P*TP*TP*TP*AP*GP*CP*GP*GP*CP*TP*AP*AP*AP*AP*G)-3' Chains: E, G, J, K
Molecule details ›
Chains: E, G, J, K
Length: 17 nucleotides
Theoretical weight: 5.32 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*(BRU)P*TP*TP*TP*AP*GP*CP*CP*GP*CP*TP*AP*AP*AP*AP*(BRU))-3' Chains: F, H, I, L
Molecule details ›
Chains: F, H, I, L
Length: 17 nucleotides
Theoretical weight: 5.29 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand BRU 12 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P3221
Unit cell:
a: 110.44Å b: 110.44Å c: 160.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.195 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
9 more

8 mentions without citation

Binding and spreading of ParB on DNA determine its biological function in Pseudomonas aeruginosa.
Kusiak et al. (2011)
7 more