› 1r4w

X-ray diffraction
2.5Å resolution

Crystal structure of Mitochondrial class kappa glutathione transferase

Released:
DOI: 10.2210/pdb1r4w/pdb
PDB entry 1r4w coloured by chain, front view.
PDB entry 1r4w coloured by chain, side view.
PDB entry 1r4w coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Parallel evolutionary pathways for glutathione transferases: structure and mechanism of the mitochondrial class kappa enzyme rGSTK1-1.
Ladner JE, Parsons JF, Rife CL, Gilliland GL, Armstrong RN
Biochemistry 43 352-61 (2004)
PMID: 14717589

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase kappa 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 226 amino acids
Theoretical weight: 25.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24473 (Residues: 1-226; Coverage: 100%)
Gene name: Gstk1
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments


Cofactor: Ligand GSH 4 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21
Unit cell:
a: 65.9Å b: 110.79Å c: 74.74Å
α: 90° β: 101.96° γ: 90°
R-values:
R R work R free
0.204 0.204 0.256
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Glutathione transferases.
Hayes et al. (2005)
21 more

2 mentions without citation

Glutathione transferases are structural and functional outliers in the thioredoxin fold.
Atkinson et al. (2009)
1 more