› 1r0l

X-ray diffraction
2.7Å resolution

1-deoxy-D-xylulose 5-phosphate reductoisomerase from zymomonas mobilis in complex with NADPH

Released:
DOI: 10.2210/pdb1r0l/pdb
PDB entry 1r0l coloured by chain, front view.
PDB entry 1r0l coloured by chain, side view.
PDB entry 1r0l coloured by chain, top view.
Source organism: Zymomonas mobilis
Primary publication:
Crystal structure of 1-deoxy-d-xylulose-5-phosphate reductoisomerase from Zymomonas mobilis at 1.9-A resolution.
Ricagno S, Grolle S, Bringer-Meyer S, Sahm H, Lindqvist Y, Schneider G
Biochim Biophys Acta 1698 37-44 (2004)
PMID: 15063313

Function and Biology Details

Reaction catalysed: 
2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194923 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-deoxy-D-xylulose 5-phosphate reductoisomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 41.89 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X5F2 (Residues: 1-388; Coverage: 100%)
Gene names: ZMO1150, dxr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 87.7Å b: 93.2Å c: 98.6Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.249 0.249 0.269
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
6 more