› 1qqj

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF MOUSE FUMARYLACETOACETATE HYDROLASE REFINED AT 1.55 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1qqj/pdb
PDB entry 1qqj coloured by chain, front view.
PDB entry 1qqj coloured by chain, side view.
PDB entry 1qqj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure and mechanism of a carbon-carbon bond hydrolase.
Timm DE, Mueller HA, Bhanumoorthy P, Harp JM, Bunick GJ
Structure 7 1023-33 (1999)
PMID: 10508789

Function and Biology Details

Reaction catalysed: 
4-fumarylacetoacetate + H(2)O = acetoacetate + fumarate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarylacetoacetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 46.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35505 (Residues: 1-419; Coverage: 100%)
Gene name: Fah
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ACT 2 x ACT
Ligand CAC 2 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 64Å b: 109.02Å c: 65.24Å
α: 90° β: 95.68° γ: 90°
R-values:
R R work R free
0.183 0.183 0.211
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Emergent mechanistic diversity of enzyme-catalysed beta-diketone cleavage.
Grogan G. (2005)
6 more

2 mentions without citation

Mutational spectrum of Mexican patients with tyrosinemia type 1: In silico modeling and predicted pathogenic effect of a novel missense FAH variant.
Ibarra-González et al. (2019)
1 more