› 1qne

X-ray diffraction
1.9Å resolution

Crystal structure of the Adenovirus major late promoter TATA box bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2).

Released:
DOI: 10.2210/pdb1qne/pdb
PDB entry 1qne coloured by chain, front view.
PDB entry 1qne coloured by chain, side view.
PDB entry 1qne coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.
Patikoglou GA, Kim JL, Sun L, Yang SH, Kodadek T, Burley SK
Genes Dev 13 3217-30 (1999)
PMID: 10617571

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114451 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TATA-box-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.4 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P28147 (Residues: 1-200; Coverage: 100%)
Gene names: At3g13445, MRP15.10, TBP1
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*A)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
DNA (5'-D(*TP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*C)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 42Å b: 147Å c: 57Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.197 0.197 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

The RNA polymerase II core promoter.
Smale et al. (2003)
27 more

6 mentions without citation

TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.
Patikoglou et al. (1999)
5 more