› 1qhz

X-ray diffraction
1.95Å resolution

NATIVE TETRAGONAL STRUCTURE OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS

Released:
DOI: 10.2210/pdb1qhz/pdb
PDB entry 1qhz coloured by chain, front view.
PDB entry 1qhz coloured by chain, side view.
PDB entry 1qhz coloured by chain, top view.
Source organism: Salipaludibacillus agaradhaerens
Primary publication:
Insights into ligand-induced conformational change in Cel5A from Bacillus agaradhaerens revealed by a catalytically active crystal form.
Varrot A, Schülein M, Davies GJ
J Mol Biol 297 819-28 (2000)
PMID: 10731432

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-131438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase 5A Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.16 KDa
Source organism: Salipaludibacillus agaradhaerens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: O85465 (Residues: 27-331; Coverage: 82%)
Gene name: cel5A
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 74.54Å b: 74.54Å c: 135.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.206
Expression system: Bacillus subtilis

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Citations

19 citation in other articles

Structural insights into the catalytic mechanism of a family 18 exo-chitinase.
van Aalten et al. (2001)
18 more

4 mentions without citation

Human sirt-1: molecular modeling and structure-function relationships of an unordered protein.
Autiero et al. (2008)
3 more