› 1qha

X-ray diffraction
2.25Å resolution

HUMAN HEXOKINASE TYPE I COMPLEXED WITH ATP ANALOGUE AMP-PNP

Released:
DOI: 10.2210/pdb1qha/pdb
PDB entry 1qha coloured by chain, front view.
PDB entry 1qha coloured by chain, side view.
PDB entry 1qha coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding of non-catalytic ATP to human hexokinase I highlights the structural components for enzyme-membrane association control.
Rosano C, Sabini E, Rizzi M, Deriu D, Murshudov G, Bianchi M, Serafini G, Magnani M, Bolognesi M
Structure 7 1427-37 (1999)
PMID: 10574795

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 917 amino acids
Theoretical weight: 102.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19367 (Residues: 1-917; Coverage: 100%)
Gene name: HK1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLC 4 x GLC
Ligand G6P 4 x G6P
Ligand MG 6 x MG
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P21
Unit cell:
a: 83.59Å b: 176.17Å c: 86.76Å
α: 90° β: 90.53° γ: 90°
R-values:
R R work R free
0.208 0.208 0.279
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family.
Kawai et al. (2005)
2 more

6 mentions without citation

Crystal structures of Escherichia coli ATP-dependent glucokinase and its complex with glucose.
Lunin et al. (2004)
5 more