› 1qfc

X-ray diffraction
2.7Å resolution

STRUCTURE OF RAT PURPLE ACID PHOSPHATASE

Released:
DOI: 10.2210/pdb1qfc/pdb
PDB entry 1qfc coloured by chain, front view.
PDB entry 1qfc coloured by chain, side view.
PDB entry 1qfc coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of a mammalian purple acid phosphatase.
Uppenberg J, Lindqvist F, Svensson C, Ek-Rylander B, Andersson G
J Mol Biol 290 201-11 (1999)
PMID: 10388567

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tartrate-resistant acid phosphatase type 5 Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.39 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29288 (Residues: 22-327; Coverage: 100%)
Gene name: Acp5
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:
Ligand NAG 1 x NAG
Ligand FE 2 x FE
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I41
Unit cell:
a: 116.38Å b: 116.38Å c: 63.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.281
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Calcineurin: form and function.
Rusnak et al. (2000)
6 more

2 mentions without citation

Examination of the skeletal proteome of the brittle star Ophiocoma wendtii reveals overall conservation of proteins but variation in spicule matrix proteins.
Seaver et al. (2015)
1 more