› 1q7s

X-ray diffraction
2Å resolution

Crystal structure of bit1

Released:
DOI: 10.2210/pdb1q7s/pdb
PDB entry 1q7s coloured by chain, front view.
PDB entry 1q7s coloured by chain, side view.
PDB entry 1q7s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a human peptidyl-tRNA hydrolase reveals a new fold and suggests basis for a bifunctional activity.
De Pereda JM, Waas WF, Jan Y, Ruoslahti E, Schimmel P, Pascual J
J Biol Chem 279 8111-5 (2004)
PMID: 14660562

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase 2, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3E5 (Residues: 63-179; Coverage: 65%)
Gene names: BIT1, CGI-147, PTH2, PTRH2
Sequence domains: Peptidyl-tRNA hydrolase PTH2
Structure domains: Bit1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 61.861Å b: 61.861Å c: 178.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.238
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Moonlighting proteins--an update.
Jeffery CJ. (2009)
5 more

5 mentions without citation

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes.
Powers et al. (2005)
4 more