› 1q5o

X-ray diffraction
2.3Å resolution

HCN2J 443-645 in the presence of cAMP, selenomethionine derivative

Released:
DOI: 10.2210/pdb1q5o/pdb
PDB entry 1q5o coloured by chain, front view.
PDB entry 1q5o coloured by chain, side view.
PDB entry 1q5o coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for modulation and agonist specificity of HCN pacemaker channels.
Zagotta WN, Olivier NB, Black KD, Young EC, Olson R, Gouaux E
Nature 425 200-5 (2003)
PMID: 12968185

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 24.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88703 (Residues: 443-645; Coverage: 24%)
Gene names: Bcng2, Hac1, Hcn2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CMP 1 x CMP
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P4212
Unit cell:
a: 95.429Å b: 95.429Å c: 50.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

65 review citations

Hyperpolarization-activated cation channels: from genes to function.
Biel et al. (2009)
64 more

28 mentions without citation

Structural insights into the mechanisms of CNBD channel function.
James et al. (2018)
27 more