› 1q43

X-ray diffraction
2Å resolution

HCN2I 443-640 in the presence of cAMP, selenomethionine derivative

Released:
DOI: 10.2210/pdb1q43/pdb
PDB entry 1q43 coloured by chain, front view.
PDB entry 1q43 coloured by chain, side view.
PDB entry 1q43 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for modulation and agonist specificity of HCN pacemaker channels.
Zagotta WN, Olivier NB, Black KD, Young EC, Olson R, Gouaux E
Nature 425 200-5 (2003)
PMID: 12968185

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-131574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 24.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88703 (Residues: 443-645; Coverage: 24%)
Gene names: Bcng2, Hac1, Hcn2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: I4
Unit cell:
a: 94.75Å b: 94.75Å c: 124.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

70 review citations

Hyperpolarization-activated cation channels: from genes to function.
Biel et al. (2009)
69 more

88 mentions without citation

The protective role of pregnancy in breast cancer.
Russo et al. (2005)
87 more